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Indian J Exp Biol ; 1990 Apr; 28(4): 341-5
Article in English | IMSEAR | ID: sea-57895

ABSTRACT

A. sobria was isolated unexpectedly on Campylobacter selective medium from abortion specimens of 16 buffaloes. All strains (AS1-AS16) required Campylobacter growth supplement (sodium pyruvate, sodium metabisulphate and ferrous sulphate) and 10% C02 atmosphere on primary isolation. They were unusually sensitive to bile salt and failed to grow on MacConkey's agar. Biochemically, these strains were homogeneous but antibiogram profile and physiological/other characters revealed a moderate heterogeneity. In vitro antibiotic sensitivity pattern showed a number of antibiotics effective whereas only a few (penicillin and co-trimoxazole) ineffective against these strains. It took 9 days for 3 x 10(8) A. sobria organisms/ml in 5 ml quantity to terminate the pregnancy in an experimentally infected buffalo via iv route. Foetal and placental lesions were classical as that of field cases. The infection evoked serum antibody response. Apparently disease-free buffalo sera were devoid of such antibodies.


Subject(s)
Abortion, Veterinary/microbiology , Aeromonas/immunology , Animals , Antibodies, Bacterial/analysis , Bile Acids and Salts/pharmacology , Buffaloes/microbiology , Culture Media , Female , Pregnancy
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